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Premium Expression Kit, PCR
| Product Name | Catalog No. | Unit | Qty | Storage |
|---|---|---|---|---|
| Premium Expression Kit, PCR | CFS-EDX-PCR | 1 set | 8 reactions | -80 |
Details
This handy kit is for small-scale trial expression tests using linear DNA templates prepared by PCR. It contains transcription premix and translation reagents to perform eight 226-µL reactions of wheat germ protein synthesis. It also contains a set of primers to produce DNA templates by the split-primer PCR method and the pEU-E01-DHFR vector as positive control.
Reaction steps
- DNA construction by split-primer PCR method
- Preparation of template DNA for transcription
- Transcription of template DNA, incubation at 37 for 6 hours
- Translation reagents preparation
- Translation of target protein by bi-layer reaction, incubation at 15 for 12-20 hours
Contents of kit
Reagents |
Specification |
Volume |
|---|---|---|
| PCR Primer Set (SPU, deSP6E01) | 1 µM, 10 nM ea. |
100 µL ea. |
| pEU-E01-DHFR vector (positive control) | 0.5 µg/µL |
10 µL |
| Transcription Buffer Premix (PCR) | - |
16 µL x 8 |
| WEPRO3240 (*1) | -
|
10 µL x 8 |
| SUB-AMIX (*2) | - |
206 µL x 8 |
| Aluminum seal | 2 strips |
|
Notes:
(*1) WEPRO1240 mixed with creatine kinase
(*2) contained in a single-break strip well, which is also used as reaction tubes
Apparatus required for reaction
@E PCR thermal cycler
E Incubator (transcription at 37, translation at 15-26)
Instruction manual
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