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Premium PLUS Expression Kit

Product Name Catalog No. Unit Qty Storage
Premium PLUS Expression Kit CFS-EDX-PLUS 1 set 8 reactions -80
Details

Unlike the current Premium Expression Kit and its PCR version, the new kit allows you to use one and the same kit with either plasmid DNA or PCR DNA templates. In addition, the high performance WEPRO7240 used in the new kit enables you to increase the yield more than twofold. Whereas, in general, plasmid DNA templates are preferable for obtaining higher yields, the yield of a protein you can expect with the new kit using a PCR DNA template is equal to or better than what you obtain with the current Premium Expression Kit using a plasmid DNA template.

Kit Comparison
  Premium
Expression Kit
Premium
Expression Kit, PCR
Premium PLUS
Expression Kit
Catalog No. CFS-EDX-S CFS-EDX-PCR CFS-EDX-PLUS
No. of reactions 8 8 8
Volume per reaction 226 µl 226 µl 226 µl
Wheat germ
extract
contained in
the premix with
creatine kinase
WEPRO1240 WEPRO1240 WEPRO7240
Vector /
primers
included in
the kit

pEU-E01-MCS vector

SPU & deSP6E01 primers

pEU-E01-MCS vector

SPU & deSP6E01 primers

Positive control DHFR DHFR DHFR
Reaction steps
  1. Preparation of plasmid DNA templates or linear DNA templates
    (split-primer PCR) for transcription
  2. Transcription using either plasmid DNA template or linear DNA template, incubation at 37 for 1 hour (plasmid DNA) or 37 for 4 hours (linear DNA)
  3. Translation reagents preparation
  4. Translation of target protein by bi-layer reaction, incubation at 15 for 20 hours

Contents of kit
Reagents
Specification
Volume
pEU-E01-MCS Vector
1.0 µg/µl
5 µL
PCR Primer Set (SPU, deSP6E01)
1 µM, 10 nM ea.
100 L ea.
pEU-E01-DHFR Vector (positive control)
1.0 µg/µl
5 µL
Transcription Premix
-
18 µL x 8
WEPRO®9240 (*1)
-
10 µL x 8
SUB-AMIX SGC (*2)
-
206 µL x 8
Aluminum seal
2 strips

Notes:
(*1) WEPRO7240 mixed with creatine kinase
(*2) contained in a single-break strip well consisting of 8 wells, which are also used as reaction tubes

Apparatus required for reaction

Incubator (Transcription at 37 , translation at 15 )

Instruction manual

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