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CFS

Reagents for Wheat Germ Cell-Free Protein Synthesis

Transcription Reagents

Product Name

& Description

Catalog No.
Specification
Qty
Storage

SP6 RNA Polymerase &

RNase Inhibitor

CFS-TSC-ENZ
80,000 units/mL
1 ml each
-20Ž
Enzymes for mRNA synthesis & RNase activity inhibition
NTP Mix
CFS-TSC-NTP
25 mM
1 ml
-20Ž
Mixture of high quality substrates for transcription
5x Transcription Buffer
CFS-TSC-5TB
5x
1 ml
-20Ž
Transcription buffer for WEPRO1240/2240 Series
5x Transcription Buffer LM CFS-TSC-5TB-LM
5x
1 ml
-20Ž
Transcription buffer for WEPRO7240/8240 Series

Translation Reagents

Wheat Germ Extract WEPRO Series
Product Name & Description
Catalog No.
Specification
Qty
Storage
WEPRO7240
CFS-WGE-7240
240 OD
1 ml
-80Ž
Newly developed wheat germ extract for high protein yields
WEPRO7240G
CFS-WGE-7240G
240 OD
1 ml
-80Ž
Wheat germ extract to produce GST-tagged proteins at high purities, prepared by removing from WEPRO7240 endogenous proteins nonspecifically adsorbing to GST-tag affinity purification resin
WEPRO7240H
CFS-WGE-7240H
240 OD
1 ml
-80Ž
Wheat germ extract to produce His-tagged proteins at high purities, prepared by removing from WEPRO7240 endogenous proteins nonspecifically adsorbing to His-tag affinity purification resin
WEPRO8240
CFS-WGE-8240
240 OD
1 ml
-80Ž
Wheat germ extract for the synthesis of labeled proteins; WEPRO7240 less amino acids
WEPRO8240G
CFS-WGE-8240G
240 OD
1 ml
-80Ž
Wheat germ extract for the synthesis of labeled proteins; WEPRO7240G less amino acids
WEPRO8240H
CFS-WGE-8240H
240 OD
1 ml
-80Ž
Wheat germ extract for the synthesis of labeled proteins; WEPRO7240H less amino acids
WEPRO1240
CFS-WGE-1240
240 OD
1 ml
-80Ž
Standard high quality wheat germ extract
WEPRO1240G
CFS-WGE-1240G
240 OD
      1 ml
-80Ž
Wheat germ extract to produce GST-tagged proteins at high purities, prepared by removing from WEPRO1240 endogenous proteins non-specifically adsorbing to GST-tag affinity purification resin
WEPRO1240H
CFS-WGE-1240H
240 OD
1 ml
-80Ž
Wheat germ extract to produce His-tagged proteins at high purities, prepared by removing from WEPRO1240 endogenous proteins non-specifically adsorbing to His-tag affinity purification resin
WEPRO2240
CFS-WGE-2240
240 OD
1 ml
-80Ž
Wheat germ extract for the synthesis of labeled proteins; WEPRO1240 less amino acids
WEPRO2240G
CFS-WGE-2240G
240 OD
1 ml
-80Ž
Wheat germ extract for the synthesis of labeled proteins; WEPRO1240G less amino acids
WEPRO2240H
CFS-WGE-2240H
240 OD
1 ml
-80Ž
Wheat germ extract for the synthesis of labeled proteins; WEPRO1240H less amino acids
Translation Buffer SUB-AMIX Series

Product Name

& Description

Catalog No.
Specification
Qty
Storage
SUB-AMIX
CFS-SUB
Set of 4 parts, 40x each
12.5 mL x 4
-20Ž
Standard translation buffer containing ATP, GTP, and 20 amino acids
SUB-AMIX NA
CFS-SUB-NA
Set of 4 parts, 40x each
12.5 mL x 4
-20Ž
Translation buffer for labeling; standard SUB-AMIX less amino acids to be used with WEPRO2240 Series
SUB-AMIX SG
CFS-SUB-SG
Set of 4 parts, 40x each
12.5 mL x 4
-20Ž
Translation buffer for Filter-and-Feed translation with WEPRO7240 Series
SUB-AMIX SG NA
CFS-SUB-SG-NA
Set of 4 parts, 40x each
12.5 mL x 4
-20Ž
Translation buffer for labeling, Filter-and-Feed translation with WEPRO8240 Series; SUB-AMIX SG less amino acids
SUB-AMIX SGC
CFS-SUB-SGC
Set of 4 parts, 40x each
12.5 mL x 4
-20 Ž
Translation buffer for Bilayer translation with WEPRO7240 Series
SUB-AMIX SGC NA
CFS-SUB-SGC-NA
Set of 4 parts, 40x each
12.5 mL x 4
-20 Ž
Translation buffer for labeling, Bilayer translation with WEPRO8240 Series; SUB-AMIX SGC less amino acids

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