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WEPRO1240G Expression Kit

Product Name Catalog No. Qty Storage
WEPRO1240G Expression Kit CFS-TRI-1240G 1 set -80/-20
Details

This kit is for producing GST-tag proteins at high purities. It contains all the transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO1240G. WEPRO1240G is a specialty wheat germ extract prepared by removing from WEPRO1240 such endogenous proteins as nonspecifically adsorb to GST-tag affinity purification resin. One kit contains sufficient amounts of reagents for one run of Protemist® DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.

Contents of kit
Reagents
Specification
Volume
WEPRO1240G
240 OD
2 mL
SUB-AMIX (S1`S4)
Set of 4 parts, 40x each
1.1 mL x 4
SP6 RNA Polymerase
80,000 units/mL
0.03 mL
RNase Inhibitor
80,000 units/mL
0.03 mL
NTP Mix
25 mM
0.2 mL
5x Transcription Buffer
5x
0.4 mL
Creatine Kinase
(to be used one time only)
20 mg/mL
0.02 mL

 

Apparatus required

@EAutomatic synthesis: Protemist® DT II
@EManual synthesis: Incubator (transcription at 37, translation at 15-26)

 

Instruction manual
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