Home > Product > WEPRO8240 (15N) Expression Kit
Product
WEPRO8240 (15N) Expression Kit
| Product Name | Catalog No. | Qty | Storage |
|---|---|---|---|
| WEPRO8240 (15N) Expression Kit | CFS-TRI-8240S15N | 1 set | -80/-20 |
Details
This kit is for producing 15N labeled proteins at high yields. Contained in the kit are the transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO8240, a specialty high yield wheat germ extract containing only traces of amino acids. Other dedicated reagents contained in the kit are 5x Transcription Buffer LM and SUB-AMIX SGC (15N), a translation buffer containing amino acids labeled with high purity 15N made by chemical synthesis. One kit contains sufficient amounts of reagents for one run of Protemist® DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates. The kit facilitates the acquisition of HSQC spectral data by NMR.
WEPRO8240 (15N) Expression kit is a product jointly developed by CellFree Science Co., Ltd., Otsuka Pharmaceutical Ltd., and Cambridge Isotope Laboratories Inc. Please feel free to contact us for further information on 13C, 15N; 13C, D, 15N, and other specific amino acid labeling,
Contents of kit
Reagents |
Specification |
Volume |
|---|---|---|
| WEPRO8240 | 240 OD |
2 mL |
| SUB-AMIX SGC (15N) (S1`S4) | Set of 4 parts, 40x each |
1.1 mL x 4 |
| SP6 RNA Polymerase | 80,000 units/mL |
0.03 mL |
| RNase Inhibitor | 80,000 units/mL |
0.03 mL |
| NTP Mix | 25 mM |
0.2 mL |
| 5x Transcription Buffer LM | 5x |
0.4 mL |
| Creatine Kinase (to be used one time only) |
20 mg/mL |
0.02 mL |
Apparatus required
@EAutomatic synthesis: Protemist® DT II
@EManual synthesis: Incubator (transcription at 37, translation at 15-26)