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Reagents for Protein Synthesis
Reagent Kits
ENDEXT® Technology contract protein synthesis services
Robotic Protein Synthesizer
Products

Reagents for Protein Synthesis
Product Name Cat. No. Product Description Outline Details
WEPRO®
Series
WEPRO1240 Lysate for protein synthesis using standard protocol Instruction Manual
WEPRO2200 Lysate for labelled protein synthesis
WEPRO1240G Lysate for GST fusion protein synthesis and subsequent purification
WEPRO1240H Lysate for His fusion protein synthesis and subsequent purification
SUB-AMIX® Translation buffer
SP6 RNA Polymerase RNA polymerase
RNase Inhibitor Rnase Inhibitor
NTPmix Transcription substrate mix
5x Transcription Buffer Transcriptioin buffer
pEU Vector Transcription vector specifically designed for wheat germ cell free system MAP Sequence


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Reagent Kits
Product Name Cat. No. Product Contents Description Details
ENDEXT® Wheat Germ Expression Premium Kit EDX-S
・WEPRO 3240
・SUB-AMIX®
・Premixed Transcription Buffer
・pEU Vector
・pEU-DHFR (for positive control)
Handy kit for primary expression trial. (Plasmid version); 226ml x 8 reactions Instruction Manual
& Protocol
newENDEXT® Wheat Germ Expression Premium Kit-PCR EDX-PCR
・WEPRO 3240
・SUB-AMIX®
・Premixed Transcription Buffer
・Two primers
・pEU-DHFR (for positive control)
Handy kit for primary expression trial (PCR version); 226ml x 8 reactions Instruction Manual
& Protocol
ENDEXT® Wheat Germ Expression S Kit TRI-S
・WEPRO1240
・SUB-AMIX®
・Transcription Buffer
・SP6 RNA Polymerase
・RNase Inhibitor
・NTPs Mix
・Creatine Kinase
Kit for protein synthesis trial using standard protocol; 6ml x 6 reactions or 226µl x 96 reaction; one 6-reaction campaign with Protemist DT Instruction Manual
& Protocol
ENDEXT® Wheat Germ Expression G Kit TRI-G
・WEPRO1240G
・SUB-AMIX®
・Transcription Buffer
・SP6 RNA Polymerase
・RNase Inhibitor
・NTPs Mix
・Creatine Kinase
Kit for GST fusion protein synthesis and purification trial; 6ml x 6 reactions or 226µl x 96 reaction; one 6-reaction campaign with Protemist DT Instruction Manual
& Protocol
newENDEXT® Wheat Germ Expression H Kit TRI-H
・WEPRO1240H
・SUB-AMIX®
・Transcription Buffer
・SP6 RNA Polymerase
・RNase Inhibitor
・NTPs Mix
・Creatine Kinase
Kit for His fusion protein synthesis and purification trial; 6ml x 6 reactions or 226μl x 96 reactions; one 6-reaction campaign with Protemist DT Instruction Manual
& Protocol


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ENDEXT® Technology contract protein synthesis services
Services Contents Details
Expression trial Using the vector provided by the customer, CFS performs a protein expression and solubility test and reports the results. Upon request, the synthesized protein sample will be returned to the customer for its own evaluation of protein activity and other properties. Detail guide
(in preparation)
Cost estimation for scaled-up contract protein production (with optional simple purification) On the basis of the result of the expression trial as described above, CFS performs a preliminary test and estimates the cost of producing a required amount of the target protein.
Contract production (with optional simple purification) Upon the customer's acceptance of the cost estimate and conditions, CFS performs contract production at that cost and conditions and deliver the product to the customer.


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Robotic Protein Synthesizer
Product Name Image Brief description Details
Desktop Model
Protemist® DT
Protemist® DT Fully automated transcription, translation, and purification (approx. 70% purification). Capable of producing approx. 0.5mg* of protein (e.g. GFP) in each of the 6 wells in an overnight campaign.

*as crude protein

Applications: antibodies production, functional analysis
Product
summary
Instruction
Manual
High-Throughput Model
GenDecoder® 1000
Gene Decoder™ 1000 Fully automated transcription and translation. Capable of producing approx. 10mg* of protein (e.g. GFP) in each of the 384 wells in an overnight campaign.

*as crude protein

Applications: high throughput screening, functional analysis
Product
summary
Instruction
Manual
Mass Production Model
Protemist®
Protemist® Fully automated transcription and translation. Capable of producing approx. 4mg* of protein (e.g. GFP) in each of the 8 tubes (or 30mg* in total) in an overnight campaign.

*as crude protein

Applications: structural analysis, antibodies production
Product
summary
Instruction
Manual

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