CESG Recommended Text for CellFree Sciences Web Page (http://www.cfsciences.com/)
CellFree Sciences' Ltd.
collaborates with the TUCenter
for Eukaryotic Structural Genomics (CESG)UT at the Unversity of Wisconsin-Madison, USA, to investigate
the potential of wheat germ cell-free protein translation as an enabling
technology for structural genomics. Two important applications of wheat germ
technology are 1) a facile assessment of the ability of a given gene to be
expressed, and, 2) the ability to produce isotopically labeled proteins
required for certain critical aspects of protein structure determination. Successful
implementation of cell-free protein expression may minimize problems in cell
harvesting, cell lysis, and pre-column manipulations. Moreover, this approach
may simplify purification efforts as the protein of interest can become the
dominant protein in the reaction mixture. Cell-free systems may also permit
labeling strategies that cannot be achieved in whole cell systems, while
potentially providing a substantial economy in the labeled material required to
provide a target protein.
CESG Publications Related to Wheat
Germ Cell-Free Technology:
Tyler, R.C., Aceti, D.J., Bingman,
C.B., Cornilescu, C.C., Fox, B.G., Frederick, R.O., Jeon, W.B., Lee, M.S.,
Newman, C.S., Peterson, F.C., Phillips, G.N., Jr., Shahan, M.N., Singh, S.,
Song, J., Sreenath, H.K., Tyler, E.M., Ulrich, E.L., Vinarov, D.A., Vojtik,
F.C., Volkman, B.F., Wrobel, R.L., Zhao, Q., and Markley, J.L. (2005) Comparison of cell-based and cell-free
protocols for producing target proteins from Arabidopsis thaliana for
structural studies, Proteins, 59, 633-643. |TUPubMedUT|
Vinarov, D.A. and Markley, J.L. (2005) High-throughput
automated platform for NMR-based structural proteomics, Expert Review
of Proteomics, 2, 49-55. |TUPubMedUT|
Vinarov, D.A.,
Lytle, B.L., Peterson, F.C., Tyler, E.M., Volkman, B.F., and Markley, J.L.
(2004) Cell-free protein production and
labeling protocol for NMR-based structural proteomics, Nature Methods,
1, 149-153. |TUPubMedUT|