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Vector - CellFree Sciences Co., Ltd.|The natural power of wheat driving science -wheat germ cell-free protein expression system-

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Vector

Dedicated expression vector that has been optimized for our wheat cell-free expression system

pEU Vector Series 

 

 

peu

pEU Vector

pEU vector is an expression vector that has been highly optimized to maximize synthetic efficiency through a wheat germ cell-free protein expression system.

By introducing bacteriophage SP6 into the transcriptional promoter and a completely artificial E01 sequence selected from a randomized nucleic acid sequence pool into the translational enhancer, an mRNA with high translational activity can be produced, and protein synthesis efficiency can be maximized. In addition to the vector outlined above, our company also offers vectors with added His-tag, GST-tag and DYKDDDDK-tag that are suited to affinity purification. You can choose which one you need depending on the situation.

Product lineup

Non-Tagged Vector

Product Number

Product Name

Position of Tag

 

Vector Map

Sequence Data

CFS-11

pEU-E01-MCS

-

-

Download

Download

His-Tagged Vector

Product Number

Product Name

Position of Tag

Protease Recognition Site

Vector Map

Sequence Data

CFS-21

pEU-E01-His-TEV-MCS-N1

N-terminus

TEV

Download

Download

CFS-22 pEU-E01-His-TEV-MCS-N2 N-terminus TEV

Download

CFS-23 pEU-E01-His-TEV-MCS-N3 N-terminus TEV

Download

CFS-24 pEU-E01-MCS-TEV-His-C1 C-terminus TEV

Download

Download

CFS-25 pEU-E01-MCS-TEV-His-C2 C-terminus TEV

Download

CFS-26 pEU-E01-MCS-TEV-His-C3 C-terminus TEV

Download

GST-Tagged Vector

Product Number

Product Name

Position of Tag

Protease Recognition Site

Vector Map

Sequence Data

CFS-31

pEU-E01-GST-PS-MCS-N1

N-terminus

PreScission

Download

Download

CFS-32 pEU-E01-GST-PS-MCS-N2 N-terminus PreScission

Download

CFS-33 pEU-E01-GST-PS-MCS-N3 N-terminus PreScission

Download

CFS-34 pEU-E01-MCS-PS-GST-C1 C-terminus PreScission

Download

Download

CFS-35 pEU-E01-MCS-PS-GST-C2 C-terminus PreScission

Download

DYKDDDDK-Tagged Vector

Product Number

Product Name

Position of Tag

Protease Recognition Site

Vector Map

Sequence Data

CFS-41

pEU-E01-DYKDDDDK-MCS-N1

N-terminus

-

Download

Download

CFS-42 pEU-E01-DYKDDDDK-MCS-N2 N-terminus -

Download

CFS-43 pEU-E01-DYKDDDDK-MCS-N3 N-terminus -

Download

 

 

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