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Vector - CellFree Sciences Co., Ltd.|The natural power of wheat driving science -wheat germ cell-free protein expression system-

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Vector

Dedicated expression vector that has been optimized for our wheat cell-free expression system

pEU Vector Series 

 

 

peu

pEU Vector

pEU vector is an expression vector that has been highly optimized to maximize synthetic efficiency through a wheat germ cell-free protein expression system.

By introducing bacteriophage SP6 into the transcriptional promoter and a completely artificial E01 sequence selected from a randomized nucleic acid sequence pool into the translational enhancer, an mRNA with high translational activity can be produced, and protein synthesis efficiency can be maximized. In addition to the vector outlined above, our company also offers vectors with added His-tag, GST-tag and FLAG-tag that are suited to affinity purification. You can choose which one you need depending on the situation.

Product lineup

Non-Tagged Vector

Product Number

Product Name

Position of Tag

Protease Recognition Site

CFS-11

pEU-E01-MCS

-

-

His-Tagged Vector

Product Number

Product Name

Position of Tag

Protease Recognition Site

CFS-21

pEU-E01-His-TEV-MCS-N1

N-terminus

TEV

CFS-22 pEU-E01-His-TEV-MCS-N2 N-terminus TEV
CFS-23 pEU-E01-His-TEV-MCS-N3 N-terminus TEV
CFS-24 pEU-E01-MCS-TEV-His-C1 C-terminus TEV
CFS-25 pEU-E01-MCS-TEV-His-C2 C-terminus TEV
CFS-26 pEU-E01-MCS-TEV-His-C3 C-terminus TEV
GST-Tagged Vector

Product Number

Product Name

Position of Tag

Protease Recognition Site

CFS-31

pEU-E01-GST-PS-MCS-N1

N-terminus

PreScission™ *

CFS-32 pEU-E01-GST-PS-MCS-N2 N-terminus PreScission™ *
CFS-33 pEU-E01-GST-PS-MCS-N3 N-terminus PreScission™ *
CFS-34 pEU-E01-MCS-PS-GST-C1 C-terminus PreScission™ *
CFS-35 pEU-E01-MCS-PS-GST-C2 C-terminus PreScission™ *
DYKDDDDK-Tagged Vector

Product Number

Product Name

Position of Tag

Protease Recognition Site

CFS-41

pEU-E01-DYKDDDDK-MCS-N1

N-terminus

-

CFS-42 pEU-E01-DYKDDDDK-MCS-N2 N-terminus -
CFS-43 pEU-E01-DYKDDDDK-MCS-N3 N-terminus -

*PreScission™ is a trademark of GE Healthcare.

 

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