|
- High success rate of expression with natural folding
- High throughput capability
- Scalability: Sub-microgram to several 10 mg order expression (by varying reaction volume)
- Effective incorporation of labeled amino acids
|
Performance Comparison Among Several Expression Systems
| |
Endext
(Wheat germ) |
Baculo
(in vivo) |
E.coli
(in vivo) |
E.coli
(in vitro) |
Yeast |
CHO |
| Coverage* |
excellent |
good |
poor |
poor |
good |
good |
Folding
and
activity |
good |
good |
poor |
poor |
poor |
good |
| Through-put |
excellent |
poor |
poor |
good |
poor |
poor |
| Typical process time ** |
1 day |
14 days |
3 - 4 days |
1 day |
3 - 4 days |
14 days |
Amino acids
labeling for NMR |
excellent |
poor |
poor |
good |
poor |
poor |
| Glycosylation |
No |
insect type |
No |
No |
yeast type |
mammalian type |
* Coverage: Kinds of proteins can be expressed (human, microbe, plant, virus).
** Process time include translation, collecting of cells, disruption of cells and recovery of soluble fraction.
|
Germ system is suitable for Structural Determination especially for NMR analysis and functional analysis of proteins.
Protein production yield with ENDEXT® Technology
| |
per 1 ml WEPRO1240 |
| Reaction method |
Bi-layer |
Repeat-batch |
| GFP, DHFR, or GUS produced |
1.6 mg |
4 mg |
|
