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Reagent Kits

Starter Kit
Product Name
Details
Premium PLUS Expression Kit

This handy trial kit is designed for testing small scale (approximately 226 µL) synthesis of your targeted proteins using our wheat germ cell-free system with either plasmid DNA templates or linear DNA templates constructed by PCR (split-primer PCR). It contains all the transcription and translation reagents, their containers, pEU vector specifically developed for the wheat germ expression system, and primer set for split-primer PCR.

Protein Research Kits
Product Name
Details
Protein Research Kit (S)

Transcription and translation reagents are premixed for easy handling and setup on a 226 µl reaction scale. Kit provides reagents to setup 24 protein expression reactions.

Protein Research Kit (G)

Premixed transcription and translation reagents for GST-tagged fusion protein expression on a 226 µl reaction scale. Kit provides reagents to setup 24 protein expression reactions.

Protein Research Kit (H)

Premixed transcription and translation reagents for His-tagged fusion protein expression on a 226 µl reaction scale. Kit provides reagents to setup 24 protein expression reactions.

Expression Kits
Product Name
Details
WEPRO7240 Expression Kit This is a high yield protein synthesis kit designed for general purpose. It contains all the transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO7240. Other dedicated reagents contained in the kit are 5x Transcription Buffer LM and SUB-AMIX SGC translation buffer. One kit contains sufficient amounts of reagents for or one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
WEPRO7240H Expression Kit This kit is designed for producing His-tag proteins at high purities and high yields. It contains all the transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO7240H. Other dedicated reagents contained in the kit are 5x Transcription Buffer LM and translation buffer SUB-AMIX SGC. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
WEPRO7240G Expression Kit This kit is designed for producing GST-tag proteins at high purities and high yields. It contains all the transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO7240G. Other dedicated reagents contained in the kit are 5x Transcription Buffer LM and SUB-AMIX SGC translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
WEPRO1240 Expression Kit This is a protein synthesis kit designed for general purpose. It contains all the transcription and translation reagents for wheat germ cell-free protein synthesis. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
WEPRO1240H Expression Kit This kit is designed for producing His-tag proteins at high purities. It contains all the transcription and translation reagents for wheat germ cell-free protein synthesis. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
WEPRO1240G Expression Kit This kit is designed for producing GST-tag proteins at high purities. It contains all the transcription and translation reagents for wheat germ cell-free protein synthesis. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
Expression Kits for Labelling
Product Name
Details
WEPRO8240 Expression Kit

This kit is designed for producing labeled proteins at high yields. It is to be used with labeled amino acids prepared by the users. Contained in the kit are transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO8240. WEPRO8240 is a specialty high yield wheat germ extract containing only traces of amino acids. Other dedicated reagents contained in the kit are 5x Transcription Buffer LM and SUB-AMIX SGC NA, an amino acid-free translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.

WEPRO8240H Expression Kit This kit is designed for producing labeled His-tag proteins at high purities and high yields. It is to be used with labeled amino acids prepared by the users. Contained in the kit are transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO8240H. Other dedicated reagents contained in the kit are 5x Transcription Buffer LM and SUB-AMIX SGC NA, an amino acid-free translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.

WEPRO8240G Expression Kit This kit is designed for producing labeled GST-tag proteins at high purities and high yields. It is to be used with labeled amino acids prepared by the users. Contained in the kit are transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO8240G. Other dedicated reagents contained in the kit are 5x Transcription Buffer LM and SUB-AMIX SGC NA, an amino acid-free translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
WEPRO2240  Expression Kit This kit is designed for producing labeled proteins. It is to be used with labeled amino acids prepared by the users. Contained in the kit are transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO2240. WEPRO2240 is a specialty wheat germ extract containing only traces of amino acids and is used in combination with SUB-AMIX NA, an amino acid-free translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
WEPRO2240H Expression Kit This kit is designed for producing labeled His-tag proteins at high purities. It is to be used with labeled amino acids prepared by the users. Contained in the kit are transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO2240H. It is used in combination with SUB-AMIX NA, an amino acid-free translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
WEPRO2240G Expression Kit

This kit is designed for producing labeled GST-tag proteins at high purities. It is to be used with labeled amino acids prepared by the users. Contained in the kit are transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO2240G. It is used in combination with SUB-AMIX NA, an amino acid-free translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates

Expression Kits for 15N Labelling
Product Name
Details
WEPRO2240 (15N) Expression Kit

This kit contains the translation buffer; SUB-AMIX (15N) which contains amino acids labelled with stable isotope. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.

WEPRO8240 (15N) Expression Kit This kit contains the translation buffer; SUB-AMIX SGC (15N) which contains amino acids labelled with stable isotope. One kit contains sufficient amounts of reagents for one run of Protemist DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.
Expression Kit for Membrane Protein
Product Name
Details
ProteoLiposome PLUS Expression Kit

This Kit is easily and rapidly for testing proteliposome synthesis of membrane protein by wheat germ cell free protein expression system. It has same format as Premium PLUS Kit which contains premixed transcription and translation reagents including prepared liposome, their containers and pEU vecto for small scale (approximately 226 É L) synthesis.

ProteoLiposome Expression Kit

This Kit provides researchers with all necessary reagents to prepare proteoliposome complexes for their proteins of interest. The kit contains lyophilized liposomes prepare from soybean asolectin for easy reaction setup.

ProteoLiposome BD Expresseion Kit

This kit provides researchers with all necessary reagents to prepare proteoliposome complexes for their proteins of interest. The kit contains lyophilized liposomes prepared from soybean asolectin for easy reaction setup. Translation method is “Bilayer-Dialysis method” which is newly developed for high yield protein production.

Expression Kit for MS
Product Name
Details
Premium PLUS Expression Kit for MS

The Premium PLUS Expression Kit for MS provides premixed reagents to perform cell-free protein expression reactions for preparation of [13C, 15N]-L-Lys and [13C, 15N]-L-Arg labeled proteins. Each kit contains the reagents to perform 16 SP6-driven mRNA expression and wheat germ protein labeling reactions.

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