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WEPRO2240G Expression Kit

Product Name Catalog No. Qty Storage
WEPRO2240G Expression Kit CFS-TRI-2240G 1 set -80/-20
Details

This kit is for producing labeled GST-tag proteins at high purities. It is to be used with labeled amino acids prepared by the user. Contained in the kit are transcription and translation reagents other than amino acids for wheat germ cell-free protein synthesis with WEPRO2240G. WEPRO2240G is a specialty wheat germ extract prepared by removing from the amino acid-less WEPRO2240 such endogenous proteins as nonspecifically adsorb to GST-tag affinity purification resin. It is used in combination with SUB-AMIX NA, an amino acid-free translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist® DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.

Contents of kit
Reagents
Specification
Volume
WEPRO2240G
240 OD
2 mL
SUB-AMIX NA (S1`S4)
Set of 4 parts, 40x each
1.1 mL x 4
SP6 RNA Polymerase
80,000 units/mL
0.03 mL
RNase Inhibitor
80,000 units/mL
0.03 mL
NTP Mix
25 mM
0.2 mL
5x Transcription Buffer
5x
0.4 mL
Creatine Kinase
(to be used one time only)
20 mg/mL
0.02 mL

 

Apparatus required

@EAutomatic synthesis: Protemist® DT II
@EManual synthesis: Incubator (transcription at 37, translation at 15-26)

 

Instruction manual
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