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WEPRO7240H Expression Kit

Product Name Catalog No. Qty Storage
WEPRO7240H Expression Kit CFS-TRI-7240H 1 set -80/-20
Details

This kit is for producing His-tag proteins at high purities and high yields. It contains all the transcription and translation reagents for wheat germ cell-free protein synthesis with WEPRO7240H. WEPRO7240H is a specialty high yield wheat germ extract prepared by removing from the high yield WEPRO7240 such endogenous proteins as non-specifically adsorb to His-tag affinity purification resin. Other dedicated reagents contained in the kit are 5x Transcription Buffer LM and SUB-AMIX SGC translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist® DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.

Contents of kit
Reagents
Specification
Volume
WEPRO7240H
240 OD
2 mL
SUB-AMIX SGC (S1`S4)
Set of 4 parts, 40x each
1.1 mL x 4
SP6 RNA Polymerase
80,000 units/mL
0.03 mL
RNase Inhibitor
80,000 units/mL
0.03 mL
NTP Mix
25 mM
0.2 mL
5x Transcription Buffer LM
5x
0.4 mL
Creatine Kinase
(to be used one time only)
20 mg/mL
0.02 mL

 

Apparatus required

@EAutomatic synthesis: Protemist® DT II
@EManual synthesis: Incubator (transcription at 37, translation at 15-26)

Instruction manual
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