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WEPRO8240G Expression Kit

Product Name Catalog No. Qty Storage
WEPRO8240G Expression Kit CFS-TRI-8240G 1 set -80/-20
Details

This kit is for producing labeled GST-tag proteins at high purities and high yields. It is to be used with labeled amino acids prepared by the user. Contained in the kit are transcription and translation reagents other than amino acids for wheat germ cell-free protein synthesis with WEPRO8240G. WEPRO8240G is a specialty high yield wheat germ extract prepared by removing from the amino acid-less, high yield WEPRO8240 such endogenous proteins as nonspecifically adsorb to GST-tag affinity purification resin. Other dedicated reagents contained in the kit are 5x Transcription Buffer LM and SUB-AMIX SGC NA, an amino acid-free translation buffer. One kit contains sufficient amounts of reagents for one run of Protemist® DT II with 6 samples (6-mL translation) or 24 samples (1.2-mL translation). It can also be used for manual synthesis with 6-, 24-, or 96-well plates.

Contents of kit
Reagents
Specification
Volume
WEPRO8240G
240 OD
2 mL
SUB-AMIX SGC NA (S1`S4)
Set of 4 parts, 40x each
1.1 mL x 4
SP6 RNA Polymerase
80,000 units/mL
0.03 mL
RNase Inhibitor
80,000 units/mL
0.03 mL
NTP Mix
25 mM
0.2 mL
5x Transcription Buffer LM
5x
0.4 mL
Creatine Kinase
(to be used one time only)
20 mg/mL
0.02 mL

 

Apparatus required

@EAutomatic synthesis: Protemist® DT II
@EManual synthesis: Incubator (transcription at 37, translation at 15-26)

 

Instruction manual
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