技術紹介

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アドバンテージ

タンパク質発現系の性能比較
  ENDEXT®
(wheat germ)		 Baculo
(in vivo)		 E.coli
(in vivo)		 E.coli
(in vitro)		 Yeast CHO
Coverage* Excellent good poor poor good good
Folding
and
Activity		 good good poor poor poor good
Through-put Excellent poor poor good poor poor
Typical
process
time**		 1day 14days 3-4days 1day 3-4days 14days
Amino
acids
labeling 		excellent poor poor good poor poor
Glycosylation No insect type No No yeast type mammalian
type

* Coverage: Kinds of proteins can be expressed; human, microbe, plant, virus

** Process time includes translation, collecting of cells, disruption of cells and recovery of soluble fraction.

Germ system is suitable for structural determination especially for NMR analysis and functional analysis of proteins.

ENDEXT®Technology の収率
  per mL  WEPRO1240 per mL  WEPRO7240
Reaction method Bilayer Filter-and-Feed Bilayer Filter-and-Feed
GFP,DHFR, or GUS produced 1.6 mg 3 mg 3.2 mg 20 mg
工程比較

基本技術と関連論文

コムギ抽出液

  1. A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes(2000)
  2. Sequence specificity and efficiency of protein N-terminal methionine elimination in wheat-embryo cell-free system(2007)

発現ベクター

  1. A set of ligation-independent in vitro translation vectors for eukaryotic protein production(2008)
  2. Selection of 5'-untranslated sequences that enhance initiation of translation in a cell-free protein synthesis system from wheat embryos(2005).

少量多品種タンパク質合成（HTS、タンパク質ライブラリー）

  1. Genome-scale, biochemical annotation method based on the wheat germ cell-free protein synthesis system(2004)
  2. A cell-free protein synthesis system for high-throughput proteomics(2002)
  3. A bilayer cell-free protein synthesis system for high-throughput screening of gene products(2002)

大量少品種タンパク質合成（抗原、構造解析）

  1. Novel protein fold discovered in the PabI family of restriction enzymes(2007)
  2. A wheat germ cell-free system is a novel way to screen protein folding and function(2003)

自動タンパク質合成機

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