コムギ無細胞タンパク質合成系の応用例（応用研究 と関連論文）

共発現（補酵素、補因子）

• Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing(2007)

マラリアタンパク合成

• Tecnical Application Note
• Wheat Germ Cell-Free System-Based Production of Malaria Proteins for　Discovery of Novel Vaccine Candidates(2008)

単鎖抗体（S-S結合）

• Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ(2003)

膜タンパク質の調製

• Characterization of ScORK28, a transmembrane functional protein receptor kinase predominantly expressed in ovaries from the wild potato species Solanum 　chacoense(2007)

核内受容体と標的核酸の解析

• DNA-binding profiling of human hormone nuclear receptors via fluorescence　correlation spectroscopy in a cell-free system(2008).

機能性タンパク質チップ

• Arabidopsis HY5 protein functions as a DNA-binding tag for purification and functional immobilization of proteins on agarose/DNA microplate(2008)

ユビキチン関連タンパク質相互作用

• A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis(2009)

カスパーゼ関連タンパク質相互作用

• The initiator caspase, caspase-10beta, and the BH-3-only molecule, Bid, demonstrate evolutionary conservation in Xenopus of their pro-apoptotic activities in the extrinsic and intrinsic pathways(2006)

ポリソーム像

• Formation of circular polyribosomes in wheat germ cell-free protein synthesis 　system(2004)

Caシグナルとストレス応答

• The wheat germ cell-free based screening of protein substrates of  calcium/calmodulin-dependent protein kinase II delta.(2008)

進化分子工学手法を用いたタンパク質高機能化

• Tolerance for random recombination of domains in prokaryotic and eukaryotic translation systems: Limited interdomain misfolding in a eukaryotic translation system(2006)

自己抗体

• Implication of allelic polymorphism of osteopontin in the development of lupus　nephritis in MRL/lpr mice(2005).

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