Campaign : Special Offer until September 30, 2022: Synthesis Services for mono-biotinylated Proteins
Special Offer until September 30, 2022:
Synthesis Services for mono-biotinylated Proteins
Biotinylation of proteins is one of the most commonly use methods for working with labeled proteins. Based on the very strong interaction between biotin and Streptavidin, biotinylated proteins can be easily used in different assay formats. Our wheat germ cell-free system allows us to easily prepare mono-biotinylated proteins during translation reactions. Find out more on our approach and services for preparing mono-biotinylated proteins on our homepage:
https://www.cfsciences.com/eg/protein-biotinylation-services
Make use of our Special Offer on every order received until September 30, 2022, for using our services to prepare a mono-biotinylated protein. This offer includes:
- Protein expression reaction on 6 ml bilayer format*,
- Protein purification using His- or GST-tag**,
- Protein QC by SDS-PAGE,
- Confirmation of protein biotinylation by HABA Test***,
- Protein stock provided in elution buffer plus glycerol****,
- Negative control reaction without template can be provided.
*CFS can use different biotin concentrations in the translation reaction depending on how the biotinylated protein will be used.
**His- or GST-tag are normally placed on C terminus.
***Using Pierce™ Biotin Quantitation Kit to determine biotinylation level.
****Buffer exchange possible on customer request.
Service fee: JPY 240,000 (EURO 1,700, USD 2,000), shipping costs not included in service fee.
CFS will provide expression vector pEU-E01-bls-TEV-MCS free of charge to enable customers to prepare their own expression template. Otherwise, CFS can arrange for template preparation by gene synthesis at a fee of JPY 200,000 (EURO 1,400; USD 1,600).
Turnaround time: 4 weeks upon availability of expression vector; turnaround time for gene synthesis and vector preparation not included.
No foreseeing statements can be made about the expected protein yields.
Contact us for more information and ordering at: This email address is being protected from spambots. You need JavaScript enabled to view it..
CellFree Sciences Co., Ltd.
