Protein-Protein Interaction assay by human protein beads array
Comprehensive protein-protein interaction analysis using proximity-dependent molecular labeling technology
Proximity-dependent molecular labeling technology is a sequence-independent technique that labels all proteins in close proximity with covalent molecules. We have achieved comprehensive protein-protein interaction analysis by using AirID, a proximity-dependent biotin-labeling enzyme, in combination with protein bead arrays.
Scheme for identification of interacting proteins by proximity-dependent biotin labeling using AirID
Protein-Protein Interaction Assay Service
Protein-Protein InteractionAssay Service with 23K Human Protein Arrays
Protein-protein interaction analysis is performed using 36 protein arrays (23,000 different human proteins are loaded), each well containing one human protein and one protein of interest fused with AirID.
Protein-protein interaction analysis using human 23K protein bead arrays and proximity-dependent biotin labeling technology
Analysis Example
Protein-Protein Interaction Assay Service by Category Array
Protein arrays (1-2 per well) containing one AirID fused protein and one protein category per well are used to perform the test.
Protein-protein interaction assay service using Diversity Arrays
Testing is performed using protein bead arrays containing one AirID-fused protein of interest and one of each of 118 human proteins per well. 【Patent Application】
Examples of Diversity Array Assay
Protein-protein interaction assay using proximity-dependent biotin labeling technology in diversity arrays
Proximity-dependent biotinyltransferase (AirID 1)) fusion TP53 or NF-κB inhibitor IκBα was used to detect protein-protein interactions by diversity array.
Budget
Feb. 2023
References
AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions.Kido K. et al.: Elife. 2020 May 11;9:e54983.
PMID: 32391793, PMCID: PMC7302878
CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis Scientific Reports volume 12, Article number: 10592 (2022)
https://www.nature.com/articles/s41598-022-14872-w
Flow of Use
Service-related Information
All proteins made using our custom services are for research purpose only; not for use in diagnostic testing and use in human.
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