|
ProteoLiposome PLUS Expression KitRecommended for first-time users and to confirm the expression of target proteins |
 |
ProteoLiposome PLUS Expression Kit
This starter kit has been designed to be user-friendly even for first-time users.
Our wheat germ cell-free expression system allows you to try synthesizing membrane protein easily.
Features:
- Membran protein has been synthesized using asolection liposome
-
No need to prepare reagents
-
Reagents necessary for each reaction are already pre-packaged in tubes
-
Synthesize proteins with just a few pipetting steps
-
Target proteins can be confirmed through SDS-PAGE/CBB staining
- Contains enough for 8 reactions on a 240 µl reaction scale
※ Since this product is premixed with reagents, rehydration and sonication of liposomes cannot be performed prior
to the reaction.
On the other hands, liposomes size increase in size by freezing and thawing. Therefore, the size of liposomes at
the time of reaction in this kit is larger than 150 nm obtained by rehydration and sonication treatment.
Recommended for:
- Users who want to confirm the expression of target membrane proteins easily using wheat germ cell-free expression system
- Users who are having difficulties with membrane protein expression using other protein expression system
This starter kit has been designed to be user-friendly even for first-time users.
Our wheat germ cell-free expression system allows you to try synthesizing all types of proteins easily.
Flow of operations
Specifications
|
Product Number |
Product Name |
Volume |
|---|---|---|
|
CFS-EDX-PLUS-PLE |
ProteoLiposome PLUS Expression Kit |
1 set |
Product Composition
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
pEU-E01-MCS |
1 µg/µL |
5 µL |
1 |
| pEU-E01-T1R1（Positive Control） | 1 µg/µL | 5 µL | 1 |
| WEPRO9L*1 | - | 21 µL | 8 |
| SUB-AMIX SGC | 1x | 210 µL | 8 |
| Transcription Premix LM *2 | 1x | 18 µL | 8 |
| Aluminum seal | - | - | 2 |
Storage Condition
-80 ℃
*1 : WEPRO9L is based on WEPRO7240, with the addition of Creatine Kinase and Asolectin Liposome.
*2 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
Resources
Related Products
|
|
ProteoLiposome BD Expression KitPerfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.
|
