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ProteoLiposome PLUS Expression Kit | CellFree Sciences

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Reagents & Kits

 

 


Can be used to synthesize membrane proteins

ProteoLiposome PLUS Expression Kit

Recommended for first-time users and to confirm the expression of target proteins

ProteoLiposome PLUS Expression Kit

ProteoLiposome PLUS Expression Kit

This starter kit has been designed to be user-friendly even for first-time users.

Our wheat germ cell-free expression system allows you to try synthesizing membrane protein easily.

Features:

  • Membran protein has been synthesized using asolection liposome
  • No need to prepare reagents

  • Reagents necessary for each reaction are already pre-packaged in tubes

  • Synthesize proteins with just a few pipetting steps

  • Target proteins can be confirmed through SDS-PAGE/CBB staining

  • Contains enough for 8 reactions on a 240 µl reaction scale

 

    ※ Since this product is premixed with reagents, rehydration and sonication of liposomes cannot be performed prior   

                       to the reaction.

                       On the other hands, liposomes size increase in size by freezing and thawing. Therefore, the size of liposomes at 

                       the time of reaction in this kit is larger than 150 nm obtained by rehydration and sonication treatment.

Recommended for:

  • Users who want to confirm the expression of target membrane proteins easily using wheat germ cell-free expression system
  • Users who are having difficulties with membrane protein expression using other protein expression system

 

 

This starter kit has been designed to be user-friendly even for first-time users.

Our wheat germ cell-free expression system allows you to try synthesizing all types of proteins easily.

 

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Flow of operations

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Specifications

Product Number

Product Name

Volume

CFS-EDX-PLUS-PLE

ProteoLiposome PLUS Expression Kit

1 set


btn order support

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

pEU-E01-MCS

1 µg/µL

5 µL

1

pEU-E01-T1R1(Positive Control)1 µg/µL5 µL1
WEPRO9L*1-21 µL8
SUB-AMIX SGC1x210 µL8
Transcription Premix LM *21x18 µL8
Aluminum seal--2
Storage Condition

 -80 ℃

 

*1 : WEPRO9L is based on WEPRO7240, with the addition of Creatine Kinase and Asolectin Liposome.

*2 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.

Product-related Information

All our products are for research purpose only; not for use in diagnostic testing and use in human.

Contact us for more information and help on the use of our products.

 

Resources

Application Notes

 

Reference List

 

Related Products

ProteoLiposome BD Kit1

ProteoLiposome BD Expression Kit

Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.

 

 

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