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Can be used to synthesize membrane proteins NanoDisc BD Kit |
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NanoDisc BD Kit
Comes as a set with reagents necessary for NanoDisc synthesis for membrane protein Perfectly suited to NanoDisc synthesis of membrane protein with good quality and yield
Features:
- NanoDisc has been synthesized using co-expression with Asolectine liposome, mRNA for MSP ( Membrane Scaffold Protein) synthesis and mRNA for target membrane protein
- Can be used for 5 reactions on 2ml of bilayer–dialysis method which has been established for proteoliposome sysnthesis
- Target proteins can be confirmed through SDS-PAGE/CBB staining.
Recommended for:
- Users who require yields of membrane proteins several hundred micrograms.
- Users who want to perform interaction analysis
- Users who want to perform structure analysis of membrane protein
This kit does not include dialysis device.
A separate Slide-A-LyzerTM MINI Dialysis Devices, 10K MWCO (Thermo Fisher Scientific Inc. P/N69570) is required.
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specifications
|
Product Number |
Product Name |
Size |
|---|---|---|
|
CFS-CNDE-BD |
NanoDisc BD Kit |
5 rxn /1 set |
Product Composition
① NanoDisc BD Kit (white box):
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
Transcription Reaction |
|||
|
SP6 RNA Polymerase |
80K unit/mL |
15 µL |
1 |
|
RNase Inhibitor |
80K unit/mL |
15 µL |
1 |
|
NTPmix |
25 mM |
120 µL |
1 |
|
5×Transcription Buffer LM |
5x |
240 µL |
1 |
|
Translation reaction |
|||
|
WEPRO7240ND |
240OD |
1mL |
1 |
|
SUB-AMIX SGC S1** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S2** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S3** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S4** |
40x |
7.6 mL |
1 |
|
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
② ND Plasmid set (white box):
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
pEU-E01-MCS-TEV-SBP-C1 |
1 mg/ml |
10ul |
1 |
|
pEU-E01-MSP |
1 mg/ml |
85ul |
1 |
|
pEU-E01-CLDN4-SBP (Control) |
1 mg/ml |
10ul |
1 |
③ SA set (white box):
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
SUB-AMIX SGC S1** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S2** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S3** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S4** |
40x |
7.6 mL |
1 |
④ Asolectin liposome, lyopphilized (green box):
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
Asolectin Liposome, lyophilized |
- |
10 mg |
6 |
Storage Condition
-80 ℃
*WEPRO7240ND is based on WEPRO7240 which dedicated for NanoDisc synthesis
**SUB-AMIX SGC S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each.
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
