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Can be used to synthesize stable isotope labeled proteins His-tagged Protein Recommendation Protein Expression Kit WEPRO8240H Core Kit |
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WEPRO8240H Core Kit
Comes as a set with reagents necessary for stable isotope labeled proteins synthesis.
Synthesises by adjusting stable isotope labeled proteins, according to the customer’s goals.
Improved removal performance against His-like proteins composed of wheat germ.
Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 Core Kit.
* This kit does not come with isotope labeled amino acids included.
If the customer wants to prepare and adjust amino acids themselves, our order-made translation buffer can be used, requiring no separate amino acid adjustment.
Features:
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Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
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Target proteins can be confirmed through SDS-PAGE/CBB staining.
Recommended for:
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Users who require yields of soluble protein ranging from a few hundred micrograms to several miligrams.
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Users who want to progress with the synthesis of dozens of samples as soluble protein simultaneously.
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Users who want to perform screening.
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Users who want to perform structure analysis.
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Users who want to perform interaction analysis.
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Users who want to perform function analysis.
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specifications
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Product Number |
Product Name |
Size |
|---|---|---|
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CFS-C8H |
WEPRO8240H Core Kit |
1 set |
Product Composition
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Reagent Name |
Concentration |
VolumeVolume |
Quantity |
|---|---|---|---|
|
WEPRO8240H |
240 OD |
1 mL |
1 |
| SUB-AMIX SGC NA S1* | 40x |
600 µL |
1 |
| SUB-AMIX SGC NA S2* | 40x | 600 µL | 1 |
| SUB-AMIX SGC NA S3* | 40x | 600 µL | 1 |
| SUB-AMIX SGC NA S4* |
40x | 600 µL | 1 |
|
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
| NTPmix |
25 mM |
120 µL |
1 |
|
5×Transcription Buffer LM |
5x |
240 µL |
1 |
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RNase Inhibitor |
80K unit/mL |
15 µL |
1 |
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SP6 RNA Polymerase |
80K unit/mL |
15 µL | 1 |
Storage Condition
-80 ℃
*SUB-AMIX SGC NA S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each.
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
Resources
Related Products
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WEPRO8240 Core KitStandard type perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein. |
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WEPRO8240G Core KitExhibits a higher level of GST-tagged fusion protein purity compared to WEPRO8240 Core Kit. |
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WEPRO8240H (15N) Core KitThe translation buffer contains stable isotope 15N labeled amino acids. Recommended for users who want to perform analysis of Heteronuclear single-quantum correlation spectroscopy (HSQC) spectrum. |
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WEPRO8240H (13C, 15N) Core KitThe translation buffer contains stable isotope 13C and 15N labeled amino acids. Recommended for users who want to perform structure analysis using Nuclear Magnetic Resonance (NMR). |
