リファレンスリスト | 株式会社セルフリーサイエンス

header jp

header en






A cell-free protein synthesis system for high-throughput proteomics.

Sawasaki T. et al.: Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14652-7.

PMID: 12409616 PMCID: PMC137474


Practical cell-free protein synthesis system using purified wheat embryos.

Takai K., Sawasaki T., Endo Y.: Nat Protoc. 2010 Feb;5(2):227-38.

PMID: 20134421


The wheat-germ cell-free expression system.

Takai K, Sawasaki T, Endo Y.: Curr Pharm Biotechnol. 2010 Apr;11(3):272-8.

PMID: 20210744


Wheat germ systems for cell-free protein expression.

Harbers M.: FEBS Lett. 2014 Aug 25;588(17):2762-73.

PMID: 24931374



Selection of 5'-untranslated sequences that enhance initiation of translation in a cell-free protein synthesis system from wheat embryos.

Kamura N. et al.: Bioorg Med Chem Lett. 2005 Dec 15;15(24):5402-6.

PMID: 16213724


A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression.

Nagy S.K. et al.: BMC Biotechnol. 2020 Mar 14;20(1):17.

PMID: 32169064, PMCID: PMC7071761



A bilayer cell-free protein synthesis system for high-throughput screening of gene products.

Sawasaki T. et al.: FEBS Lett. 2002 Mar 6;514(1):102-5.

PMID: 11904190



High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system.

Takemori N. et al.: Mol Biosyst. 2015 Feb;11(2):361-5.

PMID: 25431973


A large-scale targeted proteomics assay resource based on an in vitro human proteome.

Matsumoto M. et al.: Nat Methods. 2017 Mar;14(3):251-258.

PMID: 28267743


High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system.

Takemori N. et al.: Mol Biosyst. 2016 July 19;12(8):2389-93.

PMID: 27203355


MEERCAT: Multiplexed Efficient Cell Free Expression of Recombinant QconCATs For Large Scale Absolute Proteome Quantification.

Takemori N. et al.: Mol Cell Proteomics. 2017 Dec;16(12):2169-2183.

PMID: 29055021 PMCID: PMC5724179


A practical guide to the FLEXIQuant method.

Singh S. et al.: Methods Mol Biol. 2012;893:295-319.

PMID: 22665308


FLEXIQinase, a mass spectrometry-based assay, to unveil multikinase mechanisms.

Singh S.A. et al.: Nat Methods. 2012 Apr 8;9(5):504-8.

PMID: 22484849 PMCID: PMC3595540


FLEXITau: Quantifying Post-translational Modifications of Tau Protein in Vitro and in Human Disease.

Mair W. et al.: Anal Chem. 2016 Apr 5;88(7):3704-14.

PMID: 26877193 PMCID: PMC5808556



Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis.

Matsuoka K. et al..: Proteome Res. 2010 Aug 6;9(8):4264-73.

PMID: 20575507 PMCID: PMC2917173


Specific in situ visualization of plasma cells producing antibodies against Porphyromonas gingivalis in gingival radicular cyst: application of the enzyme-labeled antigen method.

Tsuge S. et al.: J Histochem Cytochem. 2011 Jul;59(7):673-89.

PMID: 21525188 PMCID: PMC3201162


Profiling of autoantibodies in sera of pancreatic cancer patients.

Nagayoshi Y. et al.: Ann Surg Oncol. 2014 Jun;21 Suppl 3:S459-65.

PMID: 24585405


Identification of new abscisic acid receptor agonists using a wheat cell-free based drug screening system.

Nemoto K. et al.: Sci Rep. 2018 Mar 9;8(1):4268.

PMID: 29523814 PMCID: PMC5844987

ユビキチン E3 リガーゼへの利用


Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes.

Ramadan A. et al.: BMC Plant Biol. 2015 Nov 10;15:275.

PMID: 26556605 PMCID: PMC4641371


Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins.

Takahashi H. et al.: PLoS One. 2016 Jun 1;11(6):e0156718.

PMID: 27249653 PMCID: PMC4889105


The E3 ubiquitin ligase MIB2 enhances inflammation by degrading the deubiquitinating enzyme CYLD.

Uematsu A. et al.: Nat Commun. J Biol Chem. 2019 Sep 20;294(38):14135-14148.

PMID: 31366726 PMCID: PMC6755803


The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1.

Yonezawa T. et al.: J Biol Chem. 2017 Jul 28;292(30):12528-12541

PMID: 28536267 PMCID: PMC5535027


Ubiquitin-proteasome system controls ciliogenesis at the initial step of axoneme extension.

Kasahara K. et al.: Nat Commun. 2014 Oct 1;5:5081.

PMID: 25270598 PMCID: PMC4205846


A Human DUB Protein Array for Clarification of Linkage Specificity of Polyubiquitin Chain and Application to Evaluation of Its Inhibitors.

Takahashi A. et al.: Biomedicines. 2020 Jun 4;8(6):152.

PMID: 32512835 PMCID: PMC7344921



Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay.

Takeda H. et al.: Sci Rep. 2015 Jun 10;5:11333.

PMID: 26061673 PMCID: PMC4462149


Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system.

Nozawa A. et al.: BMC Biotechnol. 2011 Apr 11;11:35.

PMID: 21481249 PMCID: PMC3090341


A cell-free method for expressing and reconstituting membrane proteins enables functional characterization of the plant receptor-like protein kinase FERONIA.

Minkoff B.B.: J Biol Chem. 2017 Apr 7;292(14):5932-5942.

PMID: 28235802 PMCID: PMC5392584


Modifications of wheat germ cell-free system for functional proteomics of plant membrane proteins.

Nozawa A., Tozawa Y.: Methods Mol Biol. 2014;1072:259-72.

PMID: 24136528


Wheat Germ Cell-Free Overexpression for the Production of Membrane Proteins.

Fogeron M.L. et al.: Methods Mol Biol. 2017;1635:91-108.

PMID: 28755365


Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The Pharmacological Analysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes.

Suzuki Y. et al.: Front Pharmacol. 2018 Feb 6;9:38.

PMID: 29467651 PMCID: PMC5808195


Engineered membrane protein antigens successfully induce antibodies against extracellular regions of claudin-5.

Hashimoto Y. et al.: Sci Rep. 2018 May 30;8(1):8383.

PMID: 29849184


Characterization of mitochondrial carrier proteins of malaria parasite Plasmodium falciparum based on in vitro translation and reconstitution.

Nozawa A. et al.: Parasitol Int. 2020 Jun 20;79:102160.

PMID: 32574727




A wheat germ cell-free system is a novel way to screen protein folding and function.

Morita E.H.: Protein Sci. 2003 Jun;12(6):1216-21.

PMID: 12761392 PMCID: PMC2323893


Cell-free protein synthesis for functional and structural studies.

Makino S. et al.: Methods Mol Biol. 2014;1091:161-78.

PMID: 24203331


Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.

Aceti D.J. et al.: J Struct Funct Genomics. 2015 Jun;16(2):67-80.

PMID: 25854603 PMCID: PMC4430420


Wheat-germ cell-free production of prion proteins for solid-state NMR structural studies.

Noirot C. et al.: N Biotechnol. 2011 Apr 30;28(3):232-8.

PMID: 20609396


NMR assignment method for amide signals with cell-free protein synthesis system.

Kohno T.: Methods Mol Biol. 2010;607:113-26.

PMID: 20204853


Cell-free protein production and labeling protocol for NMR-based structural proteomics.

Vinarov D.A. et al.: Nat Methods. 2004 Nov;1(2):149-53. Epub 2004 Oct 21.

PMID: 15782178


Structural Studies of Self-Assembled Subviral Particles: Combining Cell-Free Expression with 110 kHz MAS NMR Spectroscopy.

David G. et al.: Angew Chem Int Ed Engl. 2018 Apr 16;57(17):4787-4791

PMID: 29457857


Protein sample preparation for solid-state NMR investigations.

Lacabanne D. et al.: Prog Nucl Magn Reson Spectrosc. 2019 Feb 110:20-33.

PMID: 30803692


Protein structural biology using cell-free platform from wheat germ.

Novikova I.V. et al.: Adv Struct Chem Imaging. 2018;4(1):13.

PMID: 30524935 PMCID: PMC6244559



Functional genomics using RIKEN Arabidopsis thaliana full-length cDNAs.

Seki M., Shinozaki K.: J Plant Res. 2009 Jul;122(4):355-66.

PMID: 19412652


An integrated immunoproteomics and bioinformatics approach for the analysis of Schistosoma japonicum tegument proteins.

Chen J.H. et al.: J Proteomics. 2014 Feb 26;98:289-99.

PMID: 24448400


Human protein factory for converting the transcriptome into an in vitro-expressed proteome.

Goshima N. et al.: Nat Methods. 2008 Dec;5(12):1011-7.

PMID: 19054851



Automated cell-free protein production methods for structural studies.

Beebe E.T. et al.: Methods Mol Biol. 2014;1140:117-35.

PMID: 24590713


Development of oligomannose-coated liposome-based nasal vaccine against human parainfluenza virus type 3.

Senchi K. et al.: Front Microbiol. 2013 Nov 26;4:346.

PMID: 24324462 PMCID: PMC3840497



Cell-free protein synthesis of membrane (1,3)-β-d-glucan (curdlan) synthase: co-translational insertion in liposomes and reconstitution in nanodiscs.

Periasamy A. et al.: Biochim Biophys Acta. 2013 Feb;1828(2):743-57.

PMID: 23063656


Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents.

Genji T., Nozawa A., Tozawa Y.: Biochem Biophys Res Commun. 2010 Oct 1;400(4):638-42.

PMID: 20807510


Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex.

Goren M.A. and Fox B.G.: Protein Expr Purif. 2008 Dec;62(2):171-8.

PMID: 18765284, PMCID: PMC2586813


Apoglobin Stability Is the Major Factor Governing both Cell-free and in Vivo Expression of Holomyoglobin.

Samuel P.P.: J Biol Chem. 2015 Sep 25;290(39):23479-95.

PMID: 26205820 PMCID: PMC4583012


The quiescin sulfhydryl oxidase (hQSOX1b) tunes the expression of resistin-like molecule alpha (RELM-α or mFIZZ1) in a wheat germ cell-free extract.

Gad W. et al.: PLoS One. 2013;8(1):e55621.

PMID: 23383248 PMCID: PMC3561318


Wheat germ in vitro translation to produce one of the most toxic sodium channel specific toxins.

Gad W. et al.: Biosci Rep. 2014 Jul 29;34(4). pii: e00122.

PMID: 24924257 PMCID: PMC4114062


Functional expression, purification, characterization, and membrane reconstitution of non-structural protein 2 from hepatitis C virus.

Fogeron M.L. et al.: Protein Expr Purif. 2015 Dec;116:1-6.

PMID: 26325423


Wheat germ cell-free expression: Two detergents with a low critical micelle concentration allow for production of soluble HCV membrane proteins.

Fogeron M.L. et al.: Protein Expr Purif. 2015 Jan;105:39-46.

PMID: 25306874


Rational optimization of amber suppressor tRNAs toward efficient incorporation of a non-natural amino acid into protein in a eukaryotic wheat germ extract.

Ogawa A., Namba Y., Gakumasawa M.: Org Biomol Chem. 2016 Mar 7;14(9):2671-8.

PMID: 26832824


Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline.

Guild K. et al.: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1027-31.

PMID: 21904045 PMCID: PMC3169397


Extent and Origins of Functional Diversity in a Subfamily of Glycoside Hydrolases.

Glasgow E.M. et al.: J Mol Biol. 2019 Mar 15;431(6):1217-1233.

PMID: 30685401


Preparation of a Millimeter-Sized Supergiant Liposome That Allows for Efficient, Eukaryotic Cell-Free Translation in the Interior by Spontaneous Emulsion Transfer.

Takahashi H. and Ogawa A.: ACS Synth Biol. 2020 Jul 17;9(7):1608-1614.

PMID: 32559381



Leveraging the wheat germ cell-free protein synthesis system to accelerate malaria vaccine development. (review article)

Kanoi BN et al.: Parasitol Int. 2020 Oct 30;80:102224.

PMID: 33137499


Malaria transmission-blocking vaccines: wheat germ cell-free technology can accelerate vaccine development. (review article)

Miura K. et al.: Expert Rev Vaccines. 2019 Oct;18(10):1017-1027.

PMID: 31566026


Identification of domains within Pfs230 that elicit transmission blocking antibody responses.

Tachibana M. et al.: Vaccine. 2019 Mar 22;37(13):1799-1806.

PMID: 30824357 PMCID: PMC6708081


Discovery of Novel Plasmodium falciparum Pre-Erythrocytic Antigens for Vaccine Development.

Aguiar J.C. et al.: PLoS One. 2015 Aug 20;10(8):e0136109.

PMID: 26292257 PMCID: PMC4546230


Naturally acquired antibody responses to more than 300 Plasmodium vivax proteins in three geographic regions.

Longley R.J. et al.: PLoS Negl Trop Dis. 2017 Sep 11;11(9):e0005888.

PMID: 28892517 PMCID: PMC5614652


Identification of highly-protective combinations of Plasmodium vivax recombinant proteins for vaccine development.

França C.T. et al.: Elife. 2017 Sep 26;6. pii: e28673.

PMID: 28949293 PMCID: PMC5655538


Antibody profiles to wheat germ cell-free system synthesized Plasmodium falciparum proteins correlate with protection from symptomatic malaria in Uganda

Kanoi B.N. et al.: Vaccine. 2017 Feb 7;35(6):873-881.

PMID: 28089547


Immunoscreening of Plasmodium falciparum proteins expressed in a wheat germ cell-free system reveals a novel malaria vaccine candidate.

Morita M. et al.: Sci Rep. 2017 Apr 5;7:46086.

PMID: 28378857 PMCID: PMC5380959


当社では、グローバルヘルス技術振興基金(GHIT Fund)が支援するマラリア撲滅のためのワクチン候補とバイオマーカーを特定するプロジェクトに参加しています。




CF-PA2Vtech: a cell-free human protein array technology for antibody validation against human proteins

Morishita R. et al.: Sci Rep. 2019 Dec 18;9(1):19349.

PMID: 31852950 PMCID: PMC6920144 



Cell-free translation of biofuel enzymes.

Takasuka T.E. et al.: Methods Mol Biol. 2014;1118:71-95.

PMID: 24395410 PMCID: PMC5820533


AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions.

Kido K. et al.: Elife. 2020 May 11;9:e54983.

PMID: 32391793, PMCID: PMC7302878



Development and validation of serological markers for detecting recent Plasmodium vivax infection.

Longley R.J.; White M.T. et al.: Nat Med. 2020 May;26(5):741-749.

PMID: 32405064


In vitro translation of virally-encoded replication polyproteins to recapitulate polyprotein maturation processes.

Habersetzer J. et al.: Protein Expr Purif. 2020 Jul 15;105694.

PMID: 32681958


Whole nucleocapsid protein of SARS-CoV-2 may cause false positive results in serological assays.

Yamaoka Y. et al.: Clin Infect Dis. 2020 May 23;ciaa637.

PMID: 32445559, PMCID: PMC7314131


A cell-free enzymatic activity assay for the evaluation of HIV-1 drug resistance to protease inhibitors.

Matsunaga S. et al.: Front Microbiol. 2015 Oct 31;6:1220.

PMID: 26583013 PMCID: PMC4628118


Development of Monoclonal Antibody and Diagnostic Test for Middle East Respiratory Syndrome Coronavirus Using Cell-Free Synthesized Nucleocapsid Antigen.

Yamaoka Y. et al.: Front Microbiol. 2016 Apr 20;7:509.

PMID: 27148198, PMCID: PMC4837155




Involvement of the 3' Untranslated Region in Encapsidation of the Hepatitis C Virus.

Shi G. et al.: PLoS Pathog. 2016 Feb 11;12(2):e1005441.

PMID: 26867128 PMCID: PMC4750987


HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains.

Shibata Y. et al.: PLoS Pathog. 2017 Jan 19;13(1):e1006162.

PMID: 28103322 PMCID: PMC5283754


PIM kinases facilitate lentiviral evasion from SAMHD1 restriction via Vpx phosphorylation.

Miyakawa K. et al.: Nat Commun. 2019 Apr 23;10(1):1844.

PMID: 31015445 PMCID: PMC6479052




Members of the Plant CRK Superfamily Are Capable of Trans- and Autophosphorylation of Tyrosine Residues.

Nemoto K. et al.: J Biol Chem. 2015 Jul 3;290(27):16665-77

PMID: 25969537 PMCID: PMC4505418


Tyrosine phosphorylation of the GARU E3 ubiquitin ligase promotes gibberellin signalling by preventing GID1 degradation.

Nemoto K. et al.: Nat Commun. 2017 Oct 17;8(1):1004.

PMID: 29042542 PMCID: PMC5645313


OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability.

Ogawa S. et al.: Sci Rep. 2017 Jan 9;7:40175.

PMID: 28067270 PMCID: PMC5220304




Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop.

Kuwahara M. et al.: Nat Commun. 2016 Sep 1;7:12596.

PMID: 27581382 PMCID: PMC5025763

Copyright ©CellFree Sciences Co.,Ltd. All Rights Reserved. [login]